Influenza Virus Like Particles

Several approaches to vaccine development are reviewed including the expression of vlps in mammalian plant and insect cell expression systems. Tumpey b fang bu b john knell a robin robinson a gale smith a a novavax inc vaccine technologies 1 taft court rockville md 20850 usa b inﬂuenza branch centers for.

Pin On Science

To provide broader protection and eliminate the need for annual update of influenza vaccines biomolecular engineering of influenza virus like particles vlps to display more conserved influenza proteins such as the matrix protein m2 has been explored.

Influenza virus like particles. Virus like particles vlps are molecules that closely resemble viruses but are non infectious because they contain no viral genetic material they can be naturally occurring or synthesized through the individual expression of viral structural proteins which can then self assemble into the virus like structure. The rna genome segments are loosely encapsidated by the nucleoprotein into virus particle. Virus like particles vlps have been suggested as a promising candidate vaccine against influenza viruses.

Vlps were produced using insect cells as host cells without using a baculovirus. A secretory form of vlps consists of hemagglutinin and matrix protein 1. Zoonotic influenza viruses of h5n1 h9n2 and recently h7n9 and h10n8 subtypes have been identified as potentially pandemic 4 5.

Here we review the development of recombinant influenza virus like particles vlps as vaccines against h7n9 virus. Here we review the development of recombinant influenza virus like particles vlps as vaccines against h7n9 virus. More specifically this invention relates to the design and production of functional homotypic and heterotypic recombinant influenza virus like particles vlps comprised of recombinant structural.

Influenza viruses belong to the orthomyxoviridae family and comprise negative sense single stranded segmented rna genome. Influenza a virus like particles vlps were produced using recombinant insect cells. The vlp productivity is comparable to that of the baculovirus insect cell system.

To develop recombinant vaccine for avian influenza of the h9n2 subtype we expressed in insect cells virus like particles vlps consisting of three structural proteins of influenza a hong kong 1073 99 h9n2 virus. Avian influenza viruses represent a growing threat for an influenza pandemic. Such influenza vlps have been demonstrated to provide protective immunity in experimental animal models 12 15 19 37 44 and vlp vaccines against other diseases are in widespread clinical use 18.

Vaccine 23 2005 5751 5759 influenza virus like particles comprised of the ha na and m1 proteins of h9n2 influenza virus induce protective immune responses in balb c mice peter pushko a terrence m. Several approaches to vaccine development are reviewed including the expression of vlps in mammalian plant and insect cell expression systems.

Pin On Microbiology